polyclonal anti human eno1 (Boster Bio)
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Boster Bio
polyclonal anti human eno1
Polyclonal Anti Human Eno1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enolase+polyclonal+antibody/Human+Enolase+1%2FNon-neural+enolase+Recombinant+Protein/pmc09813957-82-7-10
Average 92 stars, based on 4 article reviews
Polyclonal Anti Human Eno1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enolase+polyclonal+antibody/Human+Enolase+1%2FNon-neural+enolase+Recombinant+Protein/pmc09813957-82-7-10
Average 92 stars, based on 4 article reviews
polyclonal anti human eno1 - by Bioz Stars,
2026-09
92/100 stars
Images
1) Product Images from "ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway"
Article Title: ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway
Journal: Frontiers in Oncology
doi: 10.3389/fonc.2022.1013035
Figure Legend Snippet: Expression of ENO1 in CRC tissues. (A) Representative immunohistochemical (IHC) images showing in situ ENO1 expression in colorectal cancer (CRC) and normal tissues (scale bar = 100 μm). (B) IHC scores of ENO1 in CRC vs normal tissues. (C) IHC scores of ENO1 in T I-II vs T III-IV tissues. (D–F) Overall survival of CRC patients with high and low expressions (D) of ENO1 with TNM staging I-II (E) and III-IV (F) . Statistical analyses were performed via two-tailed Student t test. ****, P ≤ 0.001.
Techniques Used: Expressing, Immunohistochemical staining, In Situ, Two Tailed Test
Figure Legend Snippet: Relationship between ENO1 and clinic-pathological factors in CRC patients.
Techniques Used: Expressing
Figure Legend Snippet: Results of univariate and multivariate analyses of postoperative patients’ survival by Cox’s proportional hazard model.
Techniques Used:
Figure Legend Snippet: Subgroup analysis for the influencing factor of survival among colorectal cancer patients according to ENO1 expression. Statistical analyses were performed via two-tailed Student t test. *, P ≤ 0.05.
Techniques Used: Expressing, Two Tailed Test
Figure Legend Snippet: ENO1 expression was increased in the constructed 5-fluorouracil (FU)-resistant cells. (A) Comparison of ENO1 expression between various CRC cell lines in Cancer Cell Line Encyclopedia (CCLE). (B, C) Immunoblotting results of ENO1 (B) in cell lines HCT116, SW620, HT29, SW480, and LOVO and quantitative counting (C) . (D) Cell viability comparison between HCT116 cells and 5-FU-resistant cells. (E, F) Increase in ENO1 expression (E) in 5-FU-resistant cells and the statistical graph (F) . Statistical analyses were performed via two-tailed Student t test. *,P ≤ 0.05; **,P ≤ 0.01.
Techniques Used: Expressing, Construct, Comparison, Western Blot, Two Tailed Test
Figure Legend Snippet: Knockdown of ENO1 inhibited the proliferation of 5-fluorouracil (FU)-resistant colorectal cancer (CRC) cells. (A, B) Immunoblots (A) showing ENO1 protein levels in NC and ENO1-KD group and the quantified pattern (B) , reflecting the success of transfection. (C, D) Cell viability of HCT/5-FU (C) and SW620/5-FU (D) cells in the ENO1-KD group and NC group. (E, F) Proliferation rates (E) and colony forming capacity (F) of 5-FU-resistant CRC cells in the ENO1-KD and NC groups (scale bar = 6mm). Statistical analyses were performed via two-tailed Student t test. *,P ≤ 0.05; **,P ≤ 0.01; ***,P ≤ 0.001.
Techniques Used: Knockdown, Western Blot, Transfection, Two Tailed Test
Figure Legend Snippet: ENO1 knockdown inhibited the migration of resistant cells and was associated with the EMT process. (A–C) Display of representative photographs (A) in the ENO1-KD and NC groups and the number of migration cells in HCT116/5-FU cells (scale bar = 100 μm) (B) and SW620/5-FU cells (C) . (D, F) Western blotting of E-Cadherin, N-Cadherin, and Vimentin expression levels following knockdown of ENO1 in HCT116/5-FU cells (D) and SW620/5-FU cells (F) . (E, G) The bands were quantified and are presented as mean ± SEM of three independent experiments in HCT116/5-FU cells (E) and SW620/5-FU cells (G) . Statistical analyses were performed via two-tailed Student t test. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.
Techniques Used: Knockdown, Migration, Western Blot, Expressing, Two Tailed Test
Figure Legend Snippet: The regulation of the EMT process by ENO1 is associated with energy metabolism. (A) The morphological changes during the EMT process were suppressed in the ENO1-KD group compared with those in the NC group (observed by light microscope at 200× magnification). (B, C) Western blotting of E-Cadherin, N-Cadherin, and Vimentin expression in NC and ENO1-KD groups treated with or without 20 mM 2-DG for 4 h in HCT116/5-FU cells (B) and SW620/5-FU cells (C) . (D-E) The bands were quantified and are presented as mean ± SEM of three independent experiments in HCT116/5-FU cells (D) and SW620/5-FU cells (E) . Statistical analyses were performed via two-tailed Student t test. **, P ≤ 0.01; ***, P ≤ 0.001. ns, no significance.
Techniques Used: Light Microscopy, Western Blot, Expressing, Two Tailed Test
Figure Legend Snippet: ENO1 knockdown suppresses tumor growth in colorectal cancer (CRC) in vivo . (A, B) Total body weight and tumor volume in ENO1-KD and NC groups. (C, D) Total body weight and tumor volume in ENO1-KD+5-FU and 5-FU groups. (E) Representative pictures of subcutaneous tumors harvested from the four groups. (F) The weights of tumor masses with or without treatment. (G) Stratification of mice into cluster 1 (grey) and cluster 2 (blue) according to tumor weight and ENO1 levels. (H) Percentage of NC and ENO1-KD mice in each cluster. Statistical analyses were performed via two-tailed Student t test. *, P ≤ 0.05; **, P ≤ 0.01.
Techniques Used: Knockdown, In Vivo, Two Tailed Test
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